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KMID : 0903520080510040276
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2008 Volume.51 No. 4 p.276 ~ p.280
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Effect of Ultraviolet Irradiation on Molecular Properties of Ovalbumin
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Cho Yong-Sik
Song Kyung-Bin
Yamada Koji
Han Gui-Jung
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Abstract
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To elucidate the effects of ultraviolet (UV) irradiation on molecular properties of ovalbumin, the molecular weight profile, secondary structure and tertiary structure of proteins were examined after irradiation by UV with 254 nm wavelength for 4, 8, 16 and 32 hrs, respectively. UV irradiation of protein solution caused the disruption on the native state of protein molecules. SDS-PAGE and gel permeation chromatography indicated that radiation caused initial fragmentation of polypeptide chains and as a result subsequent aggregation due to cross-linking of protein molecules. Circular dichroism (CD) study showed that UV irradiation caused the change on the secondary structure resulting in decrease of helical structure or compact denature on structure of protein depending on irradiation period. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. These results suggest that UV irradiation affect the molecular properties of ovalbumin and may have potential as a means to change the antigenicity of protein allergen.
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KEYWORD
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circular dichroism, emission intensity, molecular weight, ovalbumin, ultraviolet-irradiation
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